Expression and Prognostic Value of MCM Family Genes in Osteosarcoma

We performed a detailed cancer VS normal analysis to explore the expression and prognostic value of minichromosome maintenance (MCM) proteinsin human sarcoma. The mRNA levels MCM family genes in sarcoma were analyzed using data from ONCOMINE, GEPIA CCLE databases. KEGG database was used analyze function MCM2–7 complex DNA replication cell cycle. QRT-PCR western blot confirm differential key MCMs osteosarcoma lines. Cell Counting Kit-8 flow cytometry method detect proliferation apoptosis hFOB1.19 cells. results showed that MCM1–7 MCM10 all upregulated ONCOMINE database. MCM2, MCM4–7 highly expressed Moreover, these ten factors Furthermore, we for found MCM3, MCM4, are biomarkers Analysis datasets MCM4 MCM6–7 constituted core structure MCM2-7 hexamers. AzadC treatment overexpression significantly promoted inhibited apoptosis. present study implied MCM2–4 10 potential prognosis role may be attributable change its methylation patterns.